ELISA/assay

Human soluble endothelial protein C receptor, sEPCR ELISA Kit

MBS703733

48-Strip-Wells

565 USD

ELISA Kit

Human soluble endothelial protein C receptor, sEPCR ELISA Kit

protein C receptor, endothelial (EPCR)

Human soluble endothelial protein C receptor; sEPCR ELISA Kit; CCCA; CCD41; CD201; EPCR; MGC23024; bA42O4.2; APC receptor; CD201 antigen; OTTHUMP00000030729; activated protein C receptor; cell cycle; centrosome-associated protein; centrocyclin; endothelial protein C; protein C receptor; endothelial (EPCR)

endothelial protein C receptor; Endothelial protein C receptor; endothelial protein C receptor; APC receptor; centrocyclin; CD201 antigen; activated protein C receptor; cell cycle, centrosome-associated protein; protein C receptor, endothelial; Activated protein C receptor; APC receptor; Endothelial cell protein C receptor; CD_antigen: CD201

PROCR

PROCR; PROCR; CCCA; EPCR; CCD41; EPCR; APC receptor

EPCR_HUMAN

Human

238

This assay has high sensitivity and excellent specificity for detection of human sEPCR. No significant cross-reactivity or interference between human sEPCR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human sEPCR is typically less than 1.95 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sEPCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sEPCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sEPCR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sEPCR bound in the initial step. The color development is stopped and the intensity of the color is measured.

34335272

NP_006395.2

NM_006404.4

Q9UNN8

26,671 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Function: Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150