product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit
catalog :
MBS703729
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS703729
products type :
ELISA Kit
products full name :
Mouse matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit
products short name :
matrix metallopeptidase 8 (neutrophil collagenase)
products name syn :
Mouse matrix metalloproteinase 8/Neutrophil collagenase; MMP-8 ELISA kit; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase) ; matrix metallopeptidase 8 (neutrophil collagenase)
other names :
neutrophil collagenase; Neutrophil collagenase; neutrophil collagenase; MMP-8; collagenase 2; collagenase-2; matrix metalloproteinase 8; matrix metalloproteinase-8; matrix metallopeptidase 8; Collagenase 2; Matrix metalloproteinase-8; MMP-8
products gene name :
MMP8
other gene names :
Mmp8; Mmp8; BB138268; MMP-8
uniprot entry name :
MMP8_MOUSE
reactivity :
Mouse
sequence length :
465
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse MMP-8. No significant cross-reactivity or interference between mouse MMP-8 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse MMP-8 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
160333381
ncbi acc num :
NP_032637.3
ncbi gb acc num :
NM_008611.4
uniprot acc num :
O70138
ncbi mol weight :
53,126 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1001029); Collagen Degradation Pathway (1001030); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Matrix Metalloproteinases Pathway (198370)
uniprot summary :
Function: Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution. Catalytic activity: Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I. Cofactor: Binds 3 calcium ions per subunit . Enzyme regulation: Cannot be activated without removal of the activation peptide. Activated by matrilysin.
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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