catalog number :
MBS703726
products type :
ELISA Kit
products full name :
Human pituitary adenylate cyclase activating polypeptide, PACAP ELISA Kit
products short name :
adenylate cyclase activating polypeptide 1 (pituitary)
products name syn :
Human pituitary adenylate cyclase activating polypeptide; PACAP ELISA Kit; MGC126852; PACAP; OTTHUMP00000162201; adenylate cyclase activating polypeptide; adenylate cyclase activating polypeptide 1 (pituitary)
other names :
pituitary adenylate cyclase-activating polypeptide preproprotein; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; prepro-PACAP; adenylate cyclase activating polypeptide 1 (pituitary); PRP-48
products gene name :
ADCYAP1
other gene names :
ADCYAP1; ADCYAP1; PACAP; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38
uniprot entry name :
PACA_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ADCYAP1. No significant cross-reactivity or interference between Human ADCYAP1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 2.5 ng/ml-50 ng/ml. Sensitivity: 1 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with biotin-BSA and Avidin. Standards and samples are pipetted into the wells with a biotin-conjugated antibody specific for PACAP. After washing, PACAP antibody conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PACAP in sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001093203.1
ncbi gb acc num :
NM_001099733.1
ncbi mol weight :
18,835 Da
ncbi pathways :
Activation Of TRKA Receptors Pathway (106456); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534); NGF Signalling Via TRKA From The Plasma Membrane Pathway (106459); NGF-independant TRKA Activation Pathway (106458); SIDS Susceptibility Pathways (198901)
ncbi summary :
This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
uniprot summary :
Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.2 PublicationsManual assertion based on experiment in:Ref.6
size4 :
10x96-Strip-Wells