catalog number :
MBS703715
products type :
ELISA Kit
products full name :
Bovine Interleukin 6, IL-6 ELISA Kit
products short name :
interleukin 6 (interferon, beta 2)
products name syn :
Bovine Interleukin 6; IL-6 ELISA Kit; BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2; B-cell differentiation factor; CTL differentiation factor; OTTHUMP00000158544; hybridoma growth factor; interleukin 6; interleukin BSF-2; interleukin 6 (interferon; beta 2)
other names :
interleukin-6; Interleukin-6; interleukin-6; IL-6; interleukin 6 (interferon, beta 2)
other gene names :
IL6; IL6; IL-6
uniprot entry name :
IL6_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine IL6. No significant cross-reactivity or interference between Bovine IL6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 5 pg/ml-1000 pg/ml. Sensitivity: 2.5 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Acute phase
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-6 and Horseradish Peroxidase (HRP) conjugated IL-6. The competitive inhibition reaction is launched between with HRP labeled IL-6 and unlabeled IL-6 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-6 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776348.1
ncbi gb acc num :
NM_173923.2
ncbi mol weight :
23,759 Da
ncbi pathways :
Adipogenesis Pathway (920913); African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198553); Cellular Senescence Pathway (942958); Cellular Responses To Stress Pathway (942950); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795)
uniprot summary :
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation
size4 :
10x96-Strip-Wells