catalog number :
MBS703712
products type :
ELISA Kit
products full name :
Bovine Interleukin 10, IL-10 ELISA Kit
products short name :
interleukin 10
products name syn :
Bovine Interleukin 10; IL-10 ELISA Kit; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; interleukin 10
other names :
interleukin-10; Interleukin-10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; interleukin 10<; Cytokine synthesis inhibitory factor; CSIF
products gene name :
IL10
other gene names :
IL10; IL10; IL-10; CSIF
uniprot entry name :
IL10_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine IL-10. No significant cross-reactivity or interference between bovine IL-10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Competitive. Sensitivity: Typically less than 2.5 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-10 and Horseradish Peroxidase (HRP) conjugated IL-10. The competitive inhibition reaction is launched between with HRP labeled IL-10 and unlabeled IL-10 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-10 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776513.1
ncbi gb acc num :
NM_174088.1
ncbi mol weight :
20,438 Da
ncbi pathways :
African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Asthma Pathway (84355); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84356); Autoimmune Thyroid Disease Pathway (533)
uniprot summary :
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells
size4 :
10x96-Strip-Wells