ELISA/assay

Rat corticotropin releasing hormone, CRH ELISA Kit

MBS703710

48-Strip-Wells

605 USD

ELISA Kit

Rat corticotropin releasing hormone, CRH ELISA Kit

corticotropin releasing hormone

Rat corticotropin releasing hormone; CRH ELISA Kit; CRF; corticotropin-releasing factor; corticotropin releasing hormone

corticoliberin; Corticoliberin; corticoliberin; corticotropin-releasing factor; corticotropin-releasing hormone; corticotropin releasing hormone; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone

CRH

Crh; Crh; CRF; CRF

CRF_RAT

Rat

187

This assay has high sensitivity and excellent specificity for detection of Rat CRH. No significant cross-reactivity or interference between Rat CRH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 1.25 ng/ml-20 ng/ml. Sensitivity: 0.625 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH has been observed in association with Alzheimer's disease, and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially-fatal metabolic consequences including hypoglycemia and hepatitis. In addition to being produced in the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta. In the placenta, CRH is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, CRH may act as a trigger for parturition. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A anti-antibody specific to the antibody of CRH has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for CRH and Horseradish Peroxidase (HRP) conjugated CRH, then incubated. A competitive inhibition reaction is launched between CRH (Standards or samples) and Horseradish Peroxidase (HRP) conjugated CRH with the antibody specific for CRH. The more the amount of CRH in samples, the less antibody bound by Horseradish Peroxidase (HRP) conjugated CRH. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of CRH in the sample. The color development is stopped and the intensity of the color is measured.

13591920

NP_112281.1

NM_031019.1

P01143

20,680 Da

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Class B/2 (Secretin Family Receptors) Pathway (1010189); G Alpha (s) Signalling Events Pathway (1010194); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Long-term Depression Pathway (83478); Long-term Depression Pathway (497); Myometrial Relaxation And Contraction Pathways (198465); Serotonin And Anxiety Pathway (755431)

binds CRH receptors and mediates neuroendocrine stress response; may play a role in the consolidation of long term memory [RGD, Feb 2006]

Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.1 PublicationManual assertion based on experiment in:Ref.3

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535