ELISA/assay

Human chemerin ELISA Kit

MBS703703

96 Strip Wells

850 USD

ELISA Kit

Human chemerin ELISA Kit

retinoic acid receptor responder (tazarotene induced) 2

Human chemerin ELISA Kit; HP10433; TIG2; chemerin; retinoic acid receptor responder (tazarotene induced) 2

retinoic acid receptor responder protein 2; Retinoic acid receptor responder protein 2; retinoic acid receptor responder protein 2; chemerin; RAR-responsive protein TIG2; tazarotene-induced gene 2 protein; retinoic acid receptor responder (tazarotene induced) 2; Chemerin; RAR-responsive protein TIG2; Tazarotene-induced gene 2 protein

RARRES2

RARRES2; RARRES2; TIG2; HP10433; TIG2

RARR2_HUMAN

Human

163

This assay has high sensitivity and excellent specificity for detection of human chemerin. No significant cross-reactivity or interference between human chemerin and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human chemerin concentrations in serum, plasma, tissue homogenates. Detection Range: 31.2 ng/ml-2000 ng/ml.

Sensitivity: The minimum detectable dose of human chemerin is typically less than 7.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for chemerin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any chemerin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for chemerin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of chemerin bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506427

NP_002880.1

NM_002889.3

Q99969

18,618 Da

This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein. [provided by RefSeq, Dec 2008]

Function: Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Its dual role in inflammation and metabolism might provide a link between chronic inflammation and obesity, as well as obesity-related disorders such as type 2 diabetes and cardiovascular disease. Exhibits an antimicrobial function in the skin.7 PublicationsManual assertion based on experiment in:Ref.8

96 Strip Wells

850 USD