Mouse Aspartate Aminotransferase, AST ELISA Kit | MyBioSource MBS703700 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Aspartate Aminotransferase, AST ELISA Kit

catalog :

MBS703700

quantity :

96 Strip Wells

price :

895 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703700

products type :

ELISA Kit

products full name :

Mouse Aspartate Aminotransferase, AST ELISA Kit

products short name :

glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)

products name syn :

Mouse Aspartate Aminotransferase (AST) ELISA kit; FLJ40994; KAT4; KATIV; mitAAT; aspartate aminotransferase 2; kynurenine aminotransferase IV; glutamic-oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2)

other names :

aspartate aminotransferase, mitochondrial; Aspartate aminotransferase, mitochondrial; aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; transaminase A; fatty acid-binding protein; kynurenine aminotransferase 4; kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mitochondrial aspartate aminotransferase; plasma membrane fatty acid binding protein; plasma membrane-associated fatty acid-binding protein; glutamate oxaloacetate transaminase 2, mitochondrial; Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A

products gene name :

GOT2

other gene names :

Got2; Got2; Got-2; mAspAT; FABP-pm; AL022787; Got-2; mAspAT; FABP-1; FABPpm

uniprot entry name :

AATM_MOUSE

reactivity :

Mouse

sequence length :

430

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse GOT2. No significant cross-reactivity or interference between Mouse GOT2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 1.56 mIU/ml-100 mIU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 mIU/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Lipid transport

products description :

Introduction: Aspartate transaminase (AST) also called serum glutamic oxaloacetic transaminase (SGOT) or aspartate aminotransferase (ASAT/AAT) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells. It facilitates the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate, and vice-versa. AST is normally found in red blood cells, liver, heart, muscle tissue, pancreas, and kidneys. Low levels of AST are normally found in the blood. When body tissue or an organ such as the heart or liver is diseased or damaged, additional AST is released into the bloodstream. The amount of AST in the blood is directly related to the extent of the tissue damage. After severe damage, AST levels rise in 6 to 10 hours and remain high for about 4 days. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6754036

ncbi acc num :

NP_034455.1

ncbi gb acc num :

NM_010325.2

uniprot acc num :

P05202

ncbi mol weight :

47,411 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway 715633!!2-Oxocarboxylic Acid Metabolism Pathway 717400!!Alanine And Aspartate Metabolism Pathway 198303!!Alanine, Aspartate And Glutamate Metabolism Pathway 101155!!Alanine, Aspartate And Glutamate Metabolism Pathway 100063!!Amino Acid Metabolism Pathway 198416!!Amino Acid Synthesis And Interconversion (transamination) Pathway 970564!!Arginine And Proline Metabolism Pathway 83157!!Arginine And Proline Metabolism Pathway 323!!Biosynthesis Of Amino Acids Pathway 790952

uniprot summary :

Function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.2 PublicationsManual assertion based on experiment in:Ref.7. Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.2 PublicationsManual assertion based on experiment in:Ref.9. Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.13

size :

96 Strip Wells

price :

895 USD

more info or order :

MyBioSource product webpage