ELISA/assay

Mouse Cholecystokinin, CCK ELISA Kit

MBS703698

48-Strip-Wells

590 USD

ELISA Kit

Mouse Cholecystokinin, CCK ELISA Kit

cholecystokinin

Mouse Cholecystokinin; CCK ELISA Kit; MGC117187; OTTHUMP00000209369; cholecystokinin

cholecystokinin isoform 2; Cholecystokinin; cholecystokinin; cholecystokinin

CCK

Cck; Cck; CCK; CCK33; CCK12; CCK8

CCKN_MOUSE

Mouse

134

This assay has high sensitivity and excellent specificity for detection of Mouse CCK. No significant cross-reactivity or interference between Mouse CCK and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 31.25-2000 pg/ml. Sensitivity: 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

548962138

NP_001271437.1

NM_001284508.1

P09240

12,770 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815); Peptide Ligand-binding Receptors Pathway (1000943); Signal Transduction Pathway (970540); Signaling By GPCR Pathway (971377)

This gene encodes a member of the gastrin/cholecystokinin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the peptide hormones cholecystokinin-8, -12, -33, and others. The encoded peptides have been shown to regulate gastric acid secretion and food intake. Homozygous knockout mice for this gene exhibit impaired insulin secretion, enhanced insulin sensitivity, and resistance to obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Function: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440