ELISA/assay

Human dopamine-beta-hydroxylase, DBH ELISA Kit

MBS703682

48-Strip-Wells

565 USD

ELISA Kit

Human dopamine-beta-hydroxylase, DBH ELISA Kit

dopamine beta-hydroxylase (dopamine beta-monooxygenase)

Human dopamine-beta-hydroxylase; DBH ELISA Kit; RP11-317B10.1; DBM; dopamine beta hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase)

dopamine beta-hydroxylase; Dopamine beta-hydroxylase; dopamine beta-hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine beta-monooxygenaseCleaved into the following chain:Soluble dopamine beta-hydroxylase

DBH

DBH; DBH; DBM

DOPO_HUMAN

Human

617

This assay has high sensitivity and excellent specificity for detection of human DBH. No significant cross-reactivity or interference between human DBH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 2.5 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human DBH is typically less than 1.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human DBH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for DBH and Horseradish Peroxidase (HRP) conjugated DBH. The competitive inhibition reaction is launched between with HRP labeled DBH and unlabeled DBH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DBH in the sample. The color development is stopped and the intensity of the color is measured.

116534900

NP_000778.3

NM_000787.3

P09172

69,065 Da

Amine-derived Hormones Pathway (160983); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Monoamine Transport Pathway (198851); Tyrosine Metabolism Pathway (82959)

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]

Function: Conversion of dopamine to noradrenaline. Catalytic activity: 3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O. Cofactor: Binds 1 PQQ per subunit.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150