Human apolipoprotein A5, Apo-A5 ELISA Kit | MyBioSource MBS703672 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human apolipoprotein A5, Apo-A5 ELISA Kit

catalog :

MBS703672

quantity :

96-Strip-Wells

price :

915 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703672

products type :

ELISA Kit

products full name :

Human apolipoprotein A5, Apo-A5 ELISA Kit

products short name :

apolipoprotein A-V

products name syn :

Human apolipoprotein A5 (Apo-A5) ELISA Kit; APOAV; FLJ97995; MGC126836; MGC126838; RAP3; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V

other names :

apolipoprotein A-V; Apolipoprotein A-V; apolipoprotein A-V; apo-AV; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V; Apolipoprotein A5; Regeneration-associated protein 3

products gene name :

APOA5

other gene names :

APOA5; APOA5; RAP3; APOAV; RAP3; UNQ411/PRO773; Apo-AV; ApoA-V

uniprot entry name :

APOA5_HUMAN

reactivity :

Human

sequence length :

366

specificity :

This assay has high sensitivity and excellent specificity for detection of human Apo-A5. No significant cross-reactivity or interference between human Apo-A5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human Apo-A5 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apo-A5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

262231737

ncbi acc num :

NP_001160070.1

ncbi gb acc num :

NM_001166598.1

uniprot acc num :

Q6Q788

ncbi mol weight :

41,213 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544)

ncbi summary :

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

uniprot summary :

Function: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.3 PublicationsManual assertion based on experiment in:Ref.6

size1 :

96-Strip-Wells

price1 :

915 USD

size2 :

5x96-Strip-Wells

price2 :

2565

size3 :

10x96-Strip-Wells

price3 :

4800

more info or order :

MyBioSource product webpage