catalog number :
MBS703667
products type :
ELISA Kit
products full name :
Human cluster of differentiation 73 (CD73) ELISA Kit
products short name :
cluster of differentiation 73 (CD73)
products gene name :
CD73
specificity :
This assay has high sensitivity and excellent specificity for detection of human CD73. No significant cross-reactivity or interference between human CD73 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD73 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD73 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells