catalog number :
MBS703659
products type :
ELISA Kit
products full name :
Rat Substance P, SP ELISA Kit
products short name :
Substance P, SP
products name syn :
Rat Substance P; SP ELISA Kit; Substance P; SP
other names :
substance P, partial; Substance P; Substance P
uniprot entry name :
D0PTV7_CRYPR
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SP. No significant cross-reactivity or interference between Rat SP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ACN37865.1
ncbi mol weight :
5,522 Da
size4 :
10x96-Strip-Wells