ELISA/assay

Monkey high sensitivity C-Reactive Protein, hs-CRP ELISA Kit

MBS703656

96 Strip Wells

915 USD

ELISA Kit

Monkey high sensitivity C-Reactive Protein, hs-CRP ELISA Kit

high sensitivity C-Reactive Protein, hs-CRP

Monkey high sensitivity C-Reactive Protein; hs-CRP ELISA Kit; high sensitivity C-Reactive Protein; hs-CRP

hs-CRP

Monkey

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.625 ng/ml-40 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.156 ng/ml

Sample Volume: 50-100ul

Introduction: C-reactive protein (CRP) is a protein found in the blood in response to inflammation (an acute-phase protein). CRP is synthesized by the liver in response to factors released by fat cells (adipocytes). It is a member of the pentraxin family of proteins. CRP is a member of the class of acute-phase reactants as its levels rise dramatically during inflammatory processes occurring in the body. This increment is due to a rise in the plasma concentration of IL-6, which is produced predominantly by macrophages as well as adipocytes. CRP binds to phosphocholine on microbes. It is thought to assist in complement binding to foreign and damaged cells and enhances phagocytosis by macrophages, which express a receptor for CRP. It is also believed to play another important role in innate immunity, as an early defense system against infections. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production (and hence the severity of the precipitating cause). Serum amyloid A is a related acute-phase marker that responds rapidly in similar circumstances. To measure the CRP level, a "high-sensitivity" CRP or hs-CRP test needs to be performed and analyzed by a laboratory. This is an automated blood test designed for greater accuracy in measuring 3 low levels of CRP, which allows the physician to assess cardiovascular risk. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BDNF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BDNF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BDNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

915 USD