ELISA/assay

Rat Neuron-specific enolase, NSE ELISA Kit

MBS703638

48-Strip-Wells

605 USD

ELISA Kit

Rat Neuron-specific enolase, NSE ELISA Kit

enolase 2 (gamma, neuronal)

Rat Neuron-specific enolase; NSE ELISA Kit; NSE; 2-phospho-D-glycerate hydrolyase; enolase 2; neural enolase; neuron specific gamma enolase; neurone-specific enolase; enolase 2 (gamma; neuronal)

gamma-enolase; Gamma-enolase; gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

ENO2

Eno2; Eno2; NSE; RNEN3; Eno-2; NSE

ENOG_RAT

Rat

434

This assay has high sensitivity and excellent specificity for detection of rat NSE. No significant cross-reactivity or interference between rat NSE and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: Typically less than 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSE bound in the initial step. The color development is stopped and the intensity of the color is measured.

26023949

NP_647541.1

NM_139325.3

P07323

47,141 Da

Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Disease Pathway (1010484); Gluconeogenesis Pathway (1010554); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (1010552); Glycogen Storage Diseases Pathway (1010548)

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]

Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.1 PublicationManual assertion based on experiment in:Ref.6. Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotationHAMAP-Rule:MF_00318. Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535