ELISA/assay

Mouse Parathyroid Hormone Related Protein, PTHrP ELISA Kit

MBS703635

96 Strip Wells

895 USD

ELISA Kit

Mouse Parathyroid Hormone Related Protein, PTHrP ELISA Kit

parathyroid hormone-like hormone

Mouse Parathyroid Hormone Related Protein (PTHrP) ELISA Kit; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel; parathyroid hormone-like hormone

Parathyroid hormone-related protein; Parathyroid hormone-related protein; parathyroid hormone-related protein; PLP; PTH-rP; PTH-related peptide; parathyroid hormone-like hormone; parathyroid hormone-like protein; parathyroid hormone-related peptide; parathyroid hormone-like peptide; Parathyroid hormone-like protein; PLP

PTHLH

Pthlh; Pthlh; Pthrp; PTH-like; Pthrp; PTH-rP; PTHrP; PLP

PTHR_MOUSE

Mouse

175

This assay has high sensitivity and excellent specificity for detection of Mouse PTHLH. No significant cross-reactivity or interference between Mouse PTHLH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul

Introduction: Parathyroid hormone-related protein (or PTHrP) is a protein member of the parathyroid hormone family. It is occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous cell carcinoma). However, it also has normal functions. PTHrP acts as an endocrine, autocrine, paracrine, and intracrine hormone. It regulates endochondral bone development by maintaining the endochondral growth plate at a constant width. It also regulates epithelial-mesenchymal interactions during the formation of the mammary glands. PTHrP is critical in the intraosseous phase of tooth eruption where it acts as a signalling molecule to stimulate local bone resorption. Without PTHrP, the bony crypt surrounding the tooth follicle will not resorb, and therefore the tooth will not erupt. In the context of tooth eruption, PTHrP is secreted by the cells of the Reduced Enamel Epithelium. Besides, PTHrP is necessary for maintenance of the mammary bud. Loss of PTHrP or its receptor causes the mammary bud cell fate to change back into epidermis. In lactation, it may regulate in conjunction with the calcium sensing receptor the mobilization and transfer of calcium to the milk, as well as placental transfer of calcium. PTHrP is related in function to the "normal" parathyroid hormone. When a tumor secretes PTHrP, this can lead to hypercalcemia. As this is sometimes the first sign of the malignancy, hypercalcemia caused by PTHrP is considered a paraneoplastic phenomenon. PTHrP shares the same N-terminal end as parathyroid hormone and therefore it can bind to the same receptor, the Type I PTH receptor (PTHR1). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTHrP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for PTHrP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PTHrP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PTHrP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

131543

P22858.1

P22858

20,100 Da

Class B/2 (Secretin Family Receptors) Pathway 1000967!!Endochondral Ossification Pathway 198336!!G Alpha (s) Signalling Events Pathway 1000972!!GPCR Downstream Signaling Pathway 1000971!!GPCR Ligand Binding Pathway 1000941!!Signal Transduction Pathway 970540!!Signaling By GPCR Pathway 971377

Function: Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath.2 PublicationsManual assertion based on experiment in:Ref.3

96 Strip Wells

895 USD