ELISA/assay

human transglutaminase 2C polypeptide, TGM2 ELISA Kit

MBS703613

48-Strip-Wells

565 USD

ELISA Kit

human transglutaminase 2C polypeptide, TGM2 ELISA Kit

transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)

human transglutaminase 2C polypeptide; TGM2 ELISA Kit; RP5-1054A22.2; G-ALPHA-h; GNAH; TG2; TGC; C polypeptide; TGase C; TGase-H; protein-glutamine-gamma-glutamyltransferase; tissue transglutaminase; transglutaminase 2; transglutaminase C; transglutaminase 2 (C polypeptide; protein-glutamine-gamma-glutamyltransferase)

protein-glutamine gamma-glutamyltransferase 2 isoform a; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine gamma-glutamyltransferase 2; TG(C); TGase C; TGase H; TGase-2; TGase-H; transglutaminase C; transglutaminase H; transglutaminase-2; tissue transglutaminase; epididymis secretory protein Li 45; protein-glutamine-gamma-glutamyltransferase; C polypeptide, protein-glutamine-gamma-glutamyltransferase; transglutaminase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2

TGM2

TGM2; TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h; TG(C); TGC; TGase C; TGase H; TGase-2

TGM2_HUMAN

Human

687

This assay has high sensitivity and excellent specificity for detection of human TGM2. No significant cross-reactivity or interference between human TGM2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.7 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGM2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

39777597

NP_004604.2

NM_004613.2

P21980

77,329 Da

Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Thromboxane A2 Receptor Signaling Pathway (138060)

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Catalytic activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3. Cofactor: Binds 1 calcium ion per subunit

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150