Rat von Willebrand Factor cleaving protease, ADAMTS-13/vWF-cp ELISA Kit | MyBioSource MBS703608 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat von Willebrand Factor cleaving protease, ADAMTS-13/vWF-cp ELISA Kit

catalog :

MBS703608

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703608

products type :

ELISA Kit

products full name :

Rat von Willebrand Factor cleaving protease, ADAMTS-13/vWF-cp ELISA Kit

products short name :

ADAM metallopeptidase with thrombospondin type 1 motif, 13

products name syn :

Rat von Willebrand Factor cleaving protease; ADAMTS-13/vWF-cp ELISA Kit; C9orf8; DKFZp434C2322; FLJ42993; MGC118899; MGC118900; TTP; VWFCP; vWF-CP; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 13; vWF-cleaving protease; von Wil; ADAM metallopeptidase with thrombospondin type 1 motif; 13

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein; Von Willebrand factor-cleaving protease; A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; Von Willebrand factor-cleaving protease

products gene name :

ADAMTS13

other gene names :

ADAMTS13; ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13

uniprot entry name :

Q6QNA4_HUMAN

reactivity :

Rat

sequence length :

1371

specificity :

This assay has high sensitivity and excellent specificity for detection of rat ADAMTS-13. No significant cross-reactivity or interference between rat ADAMTS-13 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat ADAMTS-13 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

73695936

ncbi acc num :

NP_620596.2

ncbi gb acc num :

NM_139027.4

uniprot acc num :

Q6QNA4

ncbi mol weight :

6,844 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

ncbi summary :

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

uniprot summary :

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Secreted, signal peptide; Calcium-binding; Motility/polarity/chemotaxis; Protease; EC 3.4.24.87; Secreted. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen. Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding. Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; response to toxin; peptide catabolic process; cell-matrix adhesion; protein processing; proteolysis; post-translational protein modification. Disease: Thrombotic Thrombocytopenic Purpura, Congenital

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage