Human type II topoisomerase, TopoII ELISA Kit | MyBioSource MBS703605 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human type II topoisomerase, TopoII ELISA Kit

catalog :

MBS703605

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703605

products type :

ELISA Kit

products full name :

Human type II topoisomerase, TopoII ELISA Kit

products short name :

topoisomerase (DNA) II alpha 170kDa

products name syn :

Human type II topoisomerase (TopoII) ELISA Kit; TOP2; TP2A; DNA topoisomerase II; 170 kD; DNA topoisomerase II; alpha isozyme; OTTHUMP00000207108; topoisomerase (DNA) II alpha 170kDa

other names :

DNA topoisomerase 2-alpha; DNA topoisomerase 2-alpha; DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme; topoisomerase (DNA) II alpha 170kDa; DNA topoisomerase II, alpha isozyme

products gene name :

TOP2A

other gene names :

TOP2A; TOP2A; TOP2; TP2A; TOP2

uniprot entry name :

TOP2A_HUMAN

reactivity :

Human

sequence length :

1531

specificity :

This assay has high sensitivity and excellent specificity for detection of human Topoll. No significant cross-reactivity or interference between human Topoll and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml-40 ng/ml. Sensitivity: The minimum detectable dose of human Topoll is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TopoII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TopoII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TopoII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TopoII bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

19913406

ncbi acc num :

NP_001058.2

ncbi gb acc num :

NM_001067.3

uniprot acc num :

P11388

ncbi mol weight :

174,385 Da

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); G0 And Early G1 Pathway (477139); Gastric Cancer Network 1 Pathway (760635); Gastric Cancer Network 2 Pathway (760637); Integrated Pancreatic Cancer Pathway (711360); Mitotic G1-G1/S Phases Pathway (160941); RB In Cancer Pathway (920977)

ncbi summary :

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

uniprot summary :

Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.2 PublicationsManual assertion based on experiment in:Ref.31. Catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA.2 PublicationsManual assertion based on experiment in:Ref.23. Cofactor: Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ Inferred.4 PublicationsManual assertion based on experiment in:Ref.23. Enzyme regulation: Specifically inhibited by the intercalating agent amsacrine.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage