catalog number :
MBS703602
products type :
ELISA Kit
products full name :
Rat apolipoprotein A1, Apo-A1 ELISA Kit
products short name :
apolipoprotein A-I
products name syn :
Rat apolipoprotein A1 (Apo-A1) ELISA Kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; apolipoprotein A-I
other names :
apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apo-AI; apoA-I; apolipoprotein A1; Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I
products gene name :
APOA1
other gene names :
APOA1; APOA1; Apo-AI; ApoA-I; ProapoA-I
uniprot entry name :
APOA1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat Apo-A1. No significant cross-reactivity or interference between rat Apo-A1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, bronchoalveolar lavage fluid and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ug/ml -10 ug/ml. Sensitivity: Typically less than 0.039 ug/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apo-A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001095157.1
ncbi gb acc num :
NM_001101687.1
ncbi mol weight :
30,591 Da
ncbi pathways :
ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324)
uniprot summary :
Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
size4 :
10x96-Strip-Wells