Human vitronectin, VTN ELISA Kit | MyBioSource MBS703597 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human vitronectin, VTN ELISA Kit

catalog :

MBS703597

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703597

products type :

ELISA Kit

products full name :

Human vitronectin, VTN ELISA Kit

products short name :

vitronectin

products name syn :

Human vitronectin (VTN) ELISA Kit; V75; VN; VNT; complement S-protein; epibolin; serum spreading factor; somatomedin B; vitronectin (serum spreading factor; somatomedin B; complement S-protein) ; vitronectin

other names :

vitronectin; Vitronectin; vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor; vitronectin; S-protein; Serum-spreading factor; V75Cleaved into the following 3 chains:Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B

products gene name :

VTN

other gene names :

VTN; VTN; VN; V75; VNT; VN

uniprot entry name :

VTNC_HUMAN

reactivity :

Human

sequence length :

478

specificity :

This assay has high sensitivity and excellent specificity for detection of human VTN. No significant cross-reactivity or interference between human VTN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100ng/ml. Sensitivity: The minimum detectable dose of human VTN is is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

88853069

ncbi acc num :

NP_000629.3

ncbi gb acc num :

NM_000638.3

uniprot acc num :

P04004

ncbi mol weight :

54,306 Da

ncbi pathways :

Complement Cascade Pathway (106405); ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); FOXA1 Transcription Factor Network Pathway (137979); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

ncbi summary :

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage