ELISA/assay

Human phosphoglycerate mutase 2, PGAM2 ELISA Kit

MBS703596

96 Strip Wells

760 USD

ELISA Kit

Human phosphoglycerate mutase 2, PGAM2 ELISA Kit

phosphoglycerate mutase 2 (muscle)

Human phosphoglycerate mutase 2; PGAM2 ELISA Kit; GSD10; MGC88743; PGAM-M; PGAMM; BPG-dependent PGAM 2; phosphoglycerate mutase 2; phosphoglycerate mutase 2 (muscle)

phosphoglycerate mutase 2; Phosphoglycerate mutase 2; phosphoglycerate mutase 2; BPG-dependent PGAM 2; phosphoglycerate mutase isozyme M; muscle-specific phosphoglycerate mutase; phosphoglycerate mutase 2 (muscle); BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM-M

PGAM2

PGAM2; PGAM2; GSD10; PGAMM; PGAM-M; PGAMM; PGAM-M

PGAM2_HUMAN

Human

253

This assay has high sensitivity and excellent specificity for detection of Human PGAM2. No significant cross-reactivity or interference between Human PGAM2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Glycolysis

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGAM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGAM2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PGAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGAM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

50593010

NP_000281.2

NM_000290.3

P15259

28,766 Da

Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Disease Pathway 530764!!Gluconeogenesis Pathway 106204!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 413342!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 468196!!Glucose Metabolism Pathway 106199!!Glycine, Serine And Threonine Metabolism Pathway 82949

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]

Function: Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.UniRule annotationHAMAP-Rule:MF_01039. Catalytic activity: 2-phospho-D-glycerate = 3-phospho-D-glycerate.UniRule annotationHAMAP-Rule:MF_01039

96 Strip Wells

760 USD