catalog number :
MBS703591
products type :
ELISA Kit
products full name :
Rat Interleukin 21, IL-21 ELISA Kit
products short name :
interleukin 21
products name syn :
Rat Interleukin 21; IL-21 ELISA Kit; IL-21; Za11; interleukin-21 isoform; interleukin 21
other names :
interleukin-21; Interleukin-21; interleukin-21; interleukin 21
products gene name :
IL21
other gene names :
Il21; Il21; IL-21; IL-21
uniprot entry name :
IL21_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat IL21. No significant cross-reactivity or interference between Rat IL21 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001102413.1
ncbi gb acc num :
NM_001108943.2
ncbi mol weight :
17,065 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Inflammatory Bowel Disease (IBD) Pathway (842782); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488)
uniprot summary :
Function: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation
size4 :
10x96-Strip-Wells