catalog number :
MBS703571
products type :
ELISA Kit
products full name :
Rat Pancreatic Lipase, PL ELISA Kit
products short name :
[pancreatic lipase]
products name syn :
[Rat Pancreatic Lipase; PL ELISA Kit; PL; pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase; pancreatic lipase]
other names :
[pancreatic triacylglycerol lipase; Pancreatic triacylglycerol lipase; pancreatic triacylglycerol lipase; PL; triglyceride lipase; pancreatic lipase-related protein 2]
products gene name :
[PNLIP]
other gene names :
[PNLIP; PNLIP; PTL; PNLIPRP2; PL; PTL; Pancreatic lipase]
uniprot entry name :
LIPP_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat PL. No significant cross-reactivity or interference between rat PL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich (Quantitative). Detection Range: 0.312 U/ml-20 U/ml. Sensitivity: 0.078 U/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075786.1
ncbi gb acc num :
NM_001082317.1
ncbi mol weight :
51,162 Da
ncbi pathways :
Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Digestion Of Dietary Lipid Pathway (106112); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Lipid Digestion, Mobilization, And Transport Pathway (106111); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells