ELISA/assay

Human Laminin-5, LN-5 ELISA Kit

MBS703569

96 Strip Wells

850 USD

ELISA Kit

Human Laminin-5, LN-5 ELISA Kit

laminin, alpha 5

Human Laminin-5 (LN-5) ELISA Kit; RP11-157P1.6; KIAA1907; laminin alpha 5; laminin alpha-5 chain; laminin; alpha 5

laminin subunit alpha-5; Laminin subunit alpha-5; laminin subunit alpha-5; laminin alpha-5 chain; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; laminin, alpha 5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha

LAMA5

LAMA5; LAMA5; KIAA0533; KIAA1907

LAMA5_HUMAN

Human

3695

This assay has high sensitivity and excellent specificity for detection of Human LAMA5. No significant cross-reactivity or interference between Human LAMA5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 12.5 ng/ml-800 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.12 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LN-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LN-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LN-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LN-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

21264602

NP_005551.3

NM_005560.4

O15230

399,737 Da

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Degradation Of The Extracellular Matrix Pathway 576263!!ECM Proteoglycans Pathway 833812!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Extracellular Matrix Organization Pathway 576262!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

96 Strip Wells

850 USD