catalog number :
MBS703565
products type :
ELISA Kit
products full name :
Chicken Insulin-like growth factors 1, IGF-1 ELISA Kit
products short name :
insulin-like growth factor 1 (somatomedin C)
products name syn :
Chicken Insulin-like growth factors 1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)
other names :
insulin-like growth factor I preproprotein; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; insulin-like growth factor-1; insulin-like growth factor 1 (somatomedin C); Somatomedin
products gene name :
IGF1
other gene names :
IGF1; IGF1; IGF-1; IGF-I; IGF-I
uniprot entry name :
IGF1_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken IGF-1. No significant cross-reactivity or interference between chicken IGF-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 125 pg/ml - 2000 pg/ml. Sensitivity: The minimum detectable dose of chicken IGF-1 is typically less than 125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IGF-1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated IGF-1. A competitive inhibition reaction is launched between IGF-1 (Standards or samples) and Biotin-conjugated IGF-1 with the pre-coated antibody specific for IGF-1. The more amount of IGF-1 in samples, the less antibody bound by Biotin-conjugated IGF-1. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of IGF-1 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001004384.1
ncbi gb acc num :
NM_001004384.2
ncbi mol weight :
17,267 Da
ncbi pathways :
Androgen Receptor Signaling Pathway (199072); Apoptosis Pathway (672475); Focal Adhesion Pathway (119312); Focal Adhesion Pathway (478); FoxO Signaling Pathway (921354); Id Signaling Pathway (199059); Oocyte Meiosis Pathway (126917); Oocyte Meiosis Pathway (126872); Progesterone-mediated Oocyte Maturation Pathway (119322); Progesterone-mediated Oocyte Maturation Pathway (503)
uniprot summary :
Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
size4 :
10x96-Strip-Wells