Human Poly ADP ribose polymerase, PARP ELISA Kit | MyBioSource MBS703546 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Poly ADP ribose polymerase, PARP ELISA Kit

catalog :

MBS703546

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703546

products type :

ELISA Kit

products full name :

Human Poly ADP ribose polymerase, PARP ELISA Kit

products short name :

poly (ADP-ribose) polymerase 1

products name syn :

Human Poly ADP ribose polymerase; PARP ELISA Kit; RP11-125A15.2; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) ; ADP-ribosyltransferase NAD (+) ; poly (ADP-ribose) polymerase family; member 1; po; poly (ADP-ribose) polymerase 1

other names :

poly; Poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); poly (ADP-ribose) polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

products gene name :

PARP1

other gene names :

PARP1; PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1

uniprot entry name :

PARP1_HUMAN

reactivity :

Human

sequence length :

1014

specificity :

This assay has high sensitivity and excellent specificity for detection of Human PARP1. No significant cross-reactivity or interference between Human PARP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: DNA damage/DNA repair. Protein Biological Process 3: DNA damage

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

156523968

ncbi acc num :

NP_001609.2

ncbi gb acc num :

NM_001618.3

uniprot acc num :

P09874

ncbi mol weight :

113,084 Da

ncbi pathways :

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Caspase Cascade In Apoptosis Pathway (137974); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (645266); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937)

ncbi summary :

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.5 PublicationsManual assertion based on experiment in:Ref.13. Catalytic activity: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.1 PublicationManual assertion based on experiment in:Ref.27

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage