Rat Vascular Endothelial cell Growth Factor, VEGF ELISA Kit | MyBioSource MBS703527 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

catalog :

MBS703527

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS703527

products type :

ELISA Kit

products full name :

Rat Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

products short name :

[vascular endothelial growth factor A]

products name syn :

[Rat Vascular Endothelial cell Growth Factor; VEGF ELISA Kit; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A]

other names :

[vascular endothelial growth factor A isoform 3; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular permeability factor; vascular endothelial growth factor A; Vascular permeability factor; VPF]

products gene name :

[VEGFA]

other gene names :

[Vegfa; Vegfa; VPF; Vegf; VEGF-A; VEGF164; Vegf; VEGF-A; VPF]

uniprot entry name :

VEGFA_RAT

reactivity :

Rat

sequence length :

325

specificity :

This assay has high sensitivity and excellent specificity for detection of rabbit VEGF. No significant cross-reactivity or interference between rabbit VEGF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 1.56 pg/ml-100 pg/ml. Sensitivity: 0.39 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

160358797

ncbi acc num :

NP_001103804.1

ncbi gb acc num :

NM_001110334.2

uniprot acc num :

P16612

ncbi mol weight :

25,239 Da

ncbi pathways :

Axon Guidance Pathway (1009871); Bladder Cancer Pathway (83503); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (1010375); Cellular Responses To Stress Pathway (1010374); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1009870); EPH-Ephrin Signaling Pathway (1009905); EPHA-mediated Growth Cone Collapse Pathway (1009906)

ncbi summary :

This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]

uniprot summary :

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.1 PublicationManual assertion based on experiment in:Ref.5

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

420

size3 :

96-Strip-Wells

price3 :

600

size4 :

5x96-Strip-Wells

price4 :

2495

size5 :

10x96-Strip-Wells

price5 :

4715

more info or order :

MyBioSource product webpage