catalog number :
MBS703522
products type :
ELISA Kit
products full name :
Mouse terminal complement complex C5b-9, TCC C5b-9 ELISA Kit
products short name :
terminal complement complex C5b-9, TCC C5b-9
products name syn :
Mouse terminal complement complex C5b-9; TCC C5b-9 ELISA Kit; terminal complement complex C5b-9; TCC C5b-9
products gene name :
TCC C5b9
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TCC C5b-9. No significant cross-reactivity or interference between mouse TCC C5b-9 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml-50 ng/ml. Sensitivity: Typically less than 0.195 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCC C5b-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCC C5b-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCC C5b-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCC C5b-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells