catalog number :
MBS703517
products type :
ELISA Kit
products full name :
Bovine Cu/Zn-Superoxide Dismutase, Cu/Zn-SOD ELISA Kit
products short name :
superoxide dismutase 1, soluble
products name syn :
Bovine Cu/Zn-Superoxide Dismutase; Cu/Zn-SOD ELISA Kit; ALS; ALS1; IPOA; SOD; homodimer; Cu/Zn superoxide dismutase; SOD; soluble; indophenoloxidase A; superoxide dismutase; cystolic; superoxide dismutase 1; soluble
other names :
superoxide dismutase; Superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; Cu-Zn superoxide dismutase; superoxide dismutase 1, soluble<
products gene name :
SOD1
other gene names :
SOD1; SOD1; SOD1L1
uniprot entry name :
SODC_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine SOD1. No significant cross-reactivity or interference between Bovine SOD1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 15.6 ng/ml-1000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cu/Zn-SOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cu/Zn-SOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Cu/Zn-SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cu/Zn-SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_777040.1
ncbi gb acc num :
NM_174615.2
ncbi mol weight :
15,683 Da
ncbi pathways :
Amyotrophic Lateral Sclerosis (ALS) Pathway 84338!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Cellular Responses To Stress Pathway 942950!!Detoxification Of Reactive Oxygen Species Pathway 942881!!Hemostasis Pathway 1009046!!Huntington's Disease Pathway 84339!!Huntington's Disease Pathway 512!!Peroxisome Pathway 131218!!Peroxisome Pathway 131126!!Platelet Activation, Signaling And Aggregation Pathway 1009056
uniprot summary :
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2. Cofactor: Binds 1 copper ion per subunit.