product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine pulmonary surfactant-associated protein B, SP-B ELISA Kit
catalog :
MBS703513
quantity :
48-Strip-Wells
price :
590 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS703513
products type :
ELISA Kit
products full name :
Bovine pulmonary surfactant-associated protein B, SP-B ELISA Kit
products short name :
surfactant protein B
products name syn :
Bovine pulmonary surfactant-associated protein B; SP-B ELISA Kit; PSP-B; SFTB3; SFTP3; SMDP1; SP-B; OTTHUMP00000203253; Pulmonary surfactant-associated protein B; 18kD; surfactant; pulmonary-associated protein B; surfactant protein B
other names :
pulmonary surfactant-associated protein B; Pulmonary surfactant-associated protein B; pulmonary surfactant-associated protein B; SP-B; 6 kDa protein; surfactant, pulmonary-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe); surfactant protein B; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)
products gene name :
SFTPB
other gene names :
SFTPB; SFTPB; SFTP3; SP-B
uniprot entry name :
PSPB_BOVIN
reactivity :
Bovine
sequence length :
373
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine SP-B. No significant cross-reactivity or interference between bovine SP-B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.37 ng/ml-600 ng/ml. Sensitivity: The minimum detectable dose of bovine SP-B is typically less than 2.34 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
115496840
ncbi acc num :
NP_001068779.1
ncbi gb acc num :
NM_001075311.2
uniprot acc num :
P15781
ncbi mol weight :
41,060 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
size1 :
48-Strip-Wells
price1 :
590 USD
size2 :
96-Strip-Wells
price2 :
850
size3 :
5x96-Strip-Wells
price3 :
2880
size4 :
10x96-Strip-Wells
price4 :
5440
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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