ELISA/assay

Human Platelet Factor 4, PF-4 ELISA Kit

MBS703500

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Platelet Factor 4, PF-4 ELISA Kit

[platelet factor 4]

[Human Platelet Factor 4; PF-4 ELISA Kit; CXCL4; MGC138298; SCYB4; chemokine (C-X-C motif) ligand 4; platelet factor 4 (chemokine (C-X-C motif) ligand 4) ; platelet factor 4]

[platelet factor 4; Platelet factor 4; platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; platelet factor 4; C-X-C motif chemokine 4; Iroplact; Oncostatin-ACleaved into the following chain:Platelet factor 4, short form]

[PF4]

[PF4; PF4; PF-4; CXCL4; SCYB4; CXCL4; SCYB4; PF-4]

PLF4_HUMAN

Human

101

This assay has high sensitivity and excellent specificity for detection of human PF-4. No significant cross-reactivity or interference between human PF-4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: Typically less than 1.56 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4505733

NP_002610.1

NM_002619.3

P02776

10,845 Da

CXCR3-mediated Signaling Events Pathway (138011); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.1 PublicationManual assertion based on experiment in:Ref.11

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

320

96-Strip-Wells

445

5x96-Strip-Wells

1880

10x96-Strip-Wells

3540