Human lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit | MyBioSource MBS703495 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

catalog :

MBS703495

quantity :

96-Strip-Wells

price :

1025 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703495

products type :

ELISA Kit

products full name :

Human lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

products short name :

phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)

products name syn :

Human lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; LDL-PLA2; LP-PLA2; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2; ; phospholipase A2; group VII (platelet-activating factor acetylhydrolase; plasma)

other names :

platelet-activating factor acetylhydrolase; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase

products gene name :

PLA2G7

other gene names :

PLA2G7; PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2; PAFAH; PAF acetylhydrolase; gVIIA-PLA2; LDL-PLA(2)

uniprot entry name :

PAFA_HUMAN

reactivity :

Human

sequence length :

441

specificity :

This assay has high sensitivity and excellent specificity for detection of human Lp-PLA2. No significant cross-reactivity or interference between human Lp-PLA2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: 0.39 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Lp-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-PLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

270133071

ncbi acc num :

NP_001161829.1

ncbi gb acc num :

NM_001168357.1

uniprot acc num :

Q13093

ncbi mol weight :

50,077 Da

ncbi pathways :

Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Lissencephaly Gene (LIS1) In Neuronal Migration And Development Pathway (137984); Metabolic Pathways (132956); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (119538)

ncbi summary :

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]

uniprot summary :

Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

size1 :

96-Strip-Wells

price1 :

1025 USD

size2 :

5x96-Strip-Wells

price2 :

2750

size3 :

10x96-Strip-Wells

price3 :

5150

more info or order :

MyBioSource product webpage