catalog number :
MBS703493
products type :
ELISA Kit
products full name :
Bovine Ceruloplasmin, CP ELISA Kit
products short name :
Ceruloplasmin, CP
products name syn :
Bovine Ceruloplasmin; CP ELISA Kit; Ceruloplasmin; CP
other names :
ceruloplasmin, partial; Ceruloplasmin; ceruloplasmin; ceruloplasmin (ferroxidase); Ferroxidase
other gene names :
CP; CP; CP-2
uniprot entry name :
CERU_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine CP. No significant cross-reactivity or interference between bovine CP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 31.2 ng/ml -2000 ng/ml. Sensitivity: The minimum detectable dose of bovine CP is typically less than 7.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine CP concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bovine CP. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for bovine CP. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of bovine CP in samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
BAA08085.1
ncbi mol weight :
122,205 Da
ncbi pathways :
HIF-1-alpha Transcription Factor Network Pathway (138045); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)
ncbi summary :
The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]
uniprot summary :
Function: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense . Catalytic activity: 4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O. Cofactor: Binds 6 copper ions per monomer.
size4 :
10x96-Strip-Wells