human prosaposin, PSAP ELISA Kit | MyBioSource MBS703488 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

human prosaposin, PSAP ELISA Kit

catalog :

MBS703488

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS703488

products type :

ELISA Kit

products full name :

human prosaposin, PSAP ELISA Kit

products short name :

[prosaposin]

products name syn :

[human prosaposin (PSAP) Elisa kit; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1; prosaposin]

other names :

[prosaposin isoform b preproprotein; Prosaposin; prosaposin; proactivator polypeptide; sphingolipid activator protein-1; prosaposin; Proactivator polypeptideCleaved into the following 5 chains:Saposin-AAlternative name(s):Protein A]

products gene name :

[PSAP]

other gene names :

[PSAP; PSAP; GLBA; SAP1; GLBA; SAP1; CSAct; SAP-1; SAP-2]

uniprot entry name :

SAP_HUMAN

reactivity :

Human

sequence length :

527

specificity :

This assay has high sensitivity and excellent specificity for detection of human PSAP. No significant cross-reactivity or interference between human PSAP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human PSAP is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<20%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PSAP. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

110224476

ncbi acc num :

NP_001035930.1

ncbi gb acc num :

NM_001042465.1

uniprot acc num :

P07602

ncbi mol weight :

58,113 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); GPCR Ligand Binding Pathway (161020); Glycosphingolipid Metabolism Pathway (530751); Hemostasis Pathway (106028); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Peptide Ligand-binding Receptors Pathway (106358); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.2 PublicationsManual assertion based on experiment in:Ref.24

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465