catalog number :
MBS703485
products type :
ELISA Kit
products full name :
Bovine cardiac troponin I, cTn-I ELISA Kit
products short name :
cardiac troponin I (cTn-I)
products name syn :
Bovine cardiac troponin I (cTn-I) ELISA kit; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; cardiac troponin I (cTn-I)
other names :
troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; cardiac troponin I; troponin I type 3 (cardiac); troponin I, cardiac muscle-like<; Cardiac troponin I
products gene name :
cTn-I
other gene names :
LOC511094; TNNI3; TNNI3
uniprot entry name :
TNNI3_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine cTn-I. No significant cross-reactivity or interference between Bovine cTn-I and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.6ng/ml-30ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.75ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Tn-I and Horseradish Peroxidase (HRP) conjugated Tn-I, then incubated. Then substrate solutions are added to the wells, respectively. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tn-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001035607.1
ncbi gb acc num :
NM_001040517.1
ncbi mol weight :
24,054 Da
ncbi pathways :
Adrenergic Signaling In Cardiomyocytes Pathway 908260!!Adrenergic Signaling In Cardiomyocytes Pathway 909696!!Cardiac Muscle Contraction Pathway 93349!!Cardiac Muscle Contraction Pathway 93992!!Dilated Cardiomyopathy Pathway 121480!!Dilated Cardiomyopathy Pathway 121285!!Hypertrophic Cardiomyopathy (HCM) Pathway 114091!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591!!Muscle Contraction Pathway 1008955!!Striated Muscle Contraction Pathway 1008956
uniprot summary :
Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.