catalog number :
MBS703484
products type :
ELISA Kit
products full name :
Rat lipoprotein lipase, LPL ELISA Kit
products short name :
lipoprotein lipase
products name syn :
Rat lipoprotein lipase; LPL ELISA Kit; HDLCQ11; LIPD; ; lipoprotein lipase
other names :
lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase
other gene names :
Lpl; Lpl; LPL
uniprot entry name :
LIPL_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat LPL. No significant cross-reactivity or interference between Rat LPL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 7.8 pg/ml-500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95 pg/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036730.1
ncbi gb acc num :
NM_012598.2
ncbi mol weight :
53,082 Da
ncbi pathways :
Adipogenesis Pathway 198479!!Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Beta Oxidation Meta Pathway 198526!!Cholesterol Metabolism Pathway 198518!!Cholesterol Metabolism Pathway 198522!!Chylomicron-mediated Lipid Transport Pathway 1010687!!Developmental Biology Pathway 1009870!!Disease Pathway 1010484!!Diseases Associated With Visual Transduction Pathway 1010532
ncbi summary :
lipase that hydrolyzes triglycerides; may be involved in the development of development of hypertriglyceridemia [RGD, Feb 2006]
uniprot summary :
Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium . Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.