catalog number :
MBS703480
products type :
ELISA Kit
products full name :
Rat soluble E-selectin, sE-selectin ELISA Kit
products short name :
selectin E
products name syn :
Rat soluble E-selectin; sE-selectin ELISA Kit; RP1-117P20.2; CD62E; ELAM; ELAM1; ESEL; LECAM2; endothelial adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; selectin E
other names :
E-selectin; E-selectin; E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; selectin E (endothelial adhesion molecule 1); leukocyte-endothelial cell adhesion molecule 2; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD_antigen: CD62E
products gene name :
SELE
other gene names :
SELE; SELE; ELAM1; ELAM-1; LECAM2
uniprot entry name :
LYAM2_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SELE. No significant cross-reactivity or interference between Rat SELE and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sE-selectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sE-selectin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sE-selectin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sE-selectin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075781.1
ncbi gb acc num :
NM_001082312.1
ncbi mol weight :
60,347 Da
ncbi pathways :
ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Glucocorticoid Receptor Regulatory Network Pathway (138014); Malaria Pathway (152665); Malaria Pathway (152657); Thromboxane A2 Receptor Signaling Pathway (138060)
uniprot summary :
Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis
size4 :
10x96-Strip-Wells