ELISA/assay

Human Soluble protein-100, S-100 ELISA Kit

MBS703472

96 Strip Wells

895 USD

ELISA Kit

Human Soluble protein-100, S-100 ELISA Kit

S100 calcium binding protein A1

Human Soluble protein-100; S-100 ELISA Kit; S100; S100-alpha; S100A; S100 alpha; S100 calcium-binding protein A1; S100 protein; alpha polypeptide; S100 calcium binding protein A1

protein S100-A1; Protein S100-A1; protein S100-A1; S100 alpha; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide; S100 calcium binding protein A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1

S100A1

S100A1; S100A1; S100; S100A; S100-alpha; S100A

S10A1_HUMAN

Human

94

This assay has high sensitivity and excellent specificity for detection of Human S100A1. No significant cross-reactivity or interference between Human S100A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.16 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.04 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain S-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of S-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

5454032

NP_006262.1

NM_006271.1

P23297

10,546 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Function: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.1 PublicationManual assertion based on experiment in:Ref.13

96 Strip Wells

895 USD