ELISA/assay

Rat Pulmonary Surfactant-associated protein D, SP-D ELISA Kit

MBS703468

48-Strip-Wells

565 USD

ELISA Kit

Rat Pulmonary Surfactant-associated protein D, SP-D ELISA Kit

surfactant protein D

Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary; surfactant protein D

pulmonary surfactant-associated protein D; Pulmonary surfactant-associated protein D; pulmonary surfactant-associated protein D; CP4; PSP-D; lung surfactant protein D; pulmonary surfactant protein D; surfactant associated protein D; surfactant, pulmonary-associated protein D; surfactant protein D; CP4; Lung surfactant protein D

SFTPD

Sftpd; Sftpd; SPD; SP-D; Sftp4; PSP-D; SP-D

SFTPD_RAT

Rat

374

This assay has high sensitivity and excellent specificity for detection of Rat SFTPD. No significant cross-reactivity or interference between Rat SFTPD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Gaseous exchange

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SP-D and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SP-D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

7949133

NP_037010.1

NM_012878.2

P35248

37,561 Da

Phagosome Pathway (153901); Phagosome Pathway (153859)

binds carbohydrates; may play a role in defense response in the lung [RGD, Feb 2006]

Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150