ELISA/assay

Human heme oxygenase 1, HO-1 ELISA Kit

MBS703465

48-Strip-Wells

565 USD

ELISA Kit

Human heme oxygenase 1, HO-1 ELISA Kit

heme oxygenase (decycling) 1

Human heme oxygenase 1; HO-1 ELISA Kit; HO-1; HSP32; bK286B10; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heat shock protein, 32-kD; heme oxygenase (decycling) 1

HMOX1

HMOX1; HMOX1; HO-1; HSP32; HMOX1D; bK286B10; HO; HO1; HO-1

HMOX1_HUMAN

Human

288

This assay has high sensitivity and excellent specificity for detection of human HO-1 . No significant cross-reactivity or interference between human HO-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: The minimum detectable dose of human HO-1 is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HO-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HO-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HO-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HO-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504437

NP_002124.1

NM_002133.2

P09601

32,819 Da

HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Heme Degradation Pathway (160971); Iron Uptake And Transport Pathway (187191); Keap1-Nrf2 Pathway (198777); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Mineral Absorption Pathway (212237)

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150