ELISA/assay

Rat von Willebrand Factor, vWF ELISA Kit

MBS703460

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat von Willebrand Factor, vWF ELISA Kit

[von Willebrand factor]

[Rat von Willebrand Factor; vWF ELISA Kit; F8VWF; VWD; coagulation factor VIII VWF; von Willebrand factor]

[von Willebrand factor; von Willebrand factor; von Willebrand factor; von Willebrand factor]

[VWF]

[Vwf; Vwf; vWF]

VWF_RAT

Rat

430

This assay has high sensitivity and excellent specificity for detection of rat vWF. No significant cross-reactivity or interference between rat vWF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich (Quantitative). Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for vWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any vWF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for vWF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of vWF bound in the initial step. The color development is stopped and the intensity of the color is measured.

56404678

Q62935.2

Q62935

48,139 Da

Blood Clotting Cascade Pathway (198493); Cardiovascular Signaling Pathway (219769); Complement And Coagulation Cascades Pathway (198461); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692247)

human homolog is a plasma glycoprotein that mediates platelet adhesion to damaged blood vessels and stabilizes blood coagulation factor VIII [RGD, Feb 2006]

Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma By similarityManual assertion inferred from sequence similarityUniProtKB:P04275

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465