ELISA/assay

Mouse Interleukin 27, IL-27 ELISA Kit

MBS703447

96 Strip Wells

575 USD

ELISA Kit

Mouse Interleukin 27, IL-27 ELISA Kit

interleukin 27

Mouse Interleukin 27; IL-27 ELISA Kit; IL-27; IL-27A; IL27p28; IL30; MGC71873; p28; IL-27 p28 subunit; interleukin 30; interleukin-27 subunit alpha; interleukin 27

interleukin-27 subunit alpha; Interleukin-27 subunit alpha; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; interleukin 27; p28

IL27

Il27; Il27; p28; Il30; IL-27; IL-27p28; Il27a; IL-27 subunit alpha; IL-27-A; IL27-A

IL27A_MOUSE

Mouse

234

This assay has high sensitivity and excellent specificity for detection of Mouse IL27. No significant cross-reactivity or interference between Mouse IL27 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.81 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity

Introduction: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as WSX-1 (TCCR) and gp130. IL-27 binds and signals through a heterodimeric receptor complex consisting of WSX-1 and gp130, both belonging to the cytokine receptor superfamily. WSX-1 is specific for IL-27 and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. In contrast, gp130 is ubiquitously expressed by a variety of immune and non-immune cells and functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 has both pro- and anti-inflammatory properties. In response to infection, IL-27 induces monocytes and mast cells to secrete pro-inflammatory cytokines. It induces naive CD4+ T cells to proliferate and develop Th1 cell responses. IL-27 also promotes effector functions of NK cells and CD8+ T cells. As an anti-inflammatory immunomodulator, IL-27 has been found to have the ability to inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-27. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-27 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

21704110

NP_663611.1

NM_145636.1

Q8K3I6

26,542 Da

IL27-mediated Signaling Events Pathway 137929

Function: Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.15 PublicationsManual assertion based on experiment in:Ref.1

96 Strip Wells

575 USD