catalog number :
MBS703439
products type :
ELISA Kit
products full name :
Mouse Oncostatin-M, OSM ELISA Kit
products short name :
oncostatin M
products name syn :
Mouse Oncostatin-M; OSM ELISA KIT; MGC20461; ; oncostatin M
other names :
oncostatin-M; Oncostatin-M; oncostatin-M; oncostatin M
other gene names :
Osm; Osm; OncoM; OSM
uniprot entry name :
ONCM_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse OSM. No significant cross-reactivity or interference between Mouse OSM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Growth Factor. Protein Biological Process 3: Growth regulation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OSM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OSM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSM bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001013383.1
ncbi gb acc num :
NM_001013365.2
ncbi mol weight :
30,114 Da
ncbi pathways :
Adipogenesis Pathway (198299); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979)
uniprot summary :
Function: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration
size4 :
10x96-Strip-Wells