ELISA/assay

Rat High mobility group protein B1, HMGB-1 ELISA Kit

MBS703437

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat High mobility group protein B1, HMGB-1 ELISA Kit

[high-mobility group box 1]

[Rat High mobility group protein B1; HMGB-1 ELISA Kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; high-mobility group box 1]

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; HMG-1; amphoterin; high mobility group 1; heparin-binding protein p30; high mobility group protein 1; high mobility group box 1; Amphoterin; Heparin-binding protein p30; High mobility group protein 1; HMG-1]

[HMGB1]

[Hmgb1; Hmgb1; Hmg1; Ac2-008; Hmg-1; Hmg1; HMG-1]

HMGB1_RAT

Rat

215

This assay has high sensitivity and excellent specificity for detection of mouse HMGB-1. No significant cross-reactivity or interference between mouse HMGB-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMGB-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6981026

NP_037095.1

NM_012963.2

P63159

24,894 Da

Base Excision Repair Pathway (83435); Base Excision Repair Pathway (451)

heparin binding protein that facilitates neurite outgrowth [RGD, Feb 2006]

Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

575

96-Strip-Wells

835

5x96-Strip-Wells

3040

10x96-Strip-Wells

5755