ELISA/assay

Rat Brain derived neurotrophic facor, BDNF ELISA Kit

MBS703433

48-Strip-Wells

435 USD

ELISA Kit

Rat Brain derived neurotrophic facor, BDNF ELISA Kit

brain-derived neurotrophic factor

Rat Brain derived neurotrophic facor; BDNF ELISA Kit; MGC34632; neurotrophin; brain-derived neurotrophic factor

brain-derived neurotrophic factor isoform 1; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; brain derived neurotrophic factor; brain derived neurothrophic factor; brain-derived neurotrophic factor

BDNF

Bdnf; Bdnf; BDNF

BDNF_RAT

Rat

362

This assay has high sensitivity and excellent specificity for detection of rat BDNF. No significant cross-reactivity or interference between rat BDNF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat BDNF is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

397174764

NP_001257559.1

NM_001270630.1

P23363

28,109 Da

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Brain Derived Neurotrophic Factor Pathway (755433); Cocaine Addiction Pathway (546271); Cocaine Addiction Pathway (546273); Huntington's Disease Pathway (83492); Huntington's Disease Pathway (512); MAPK Signaling Pathway (198496); MAPK Signaling Pathway (83440); MAPK Signaling Pathway (456)

plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]

Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS

48-Strip-Wells

435 USD

96-Strip-Wells

615

5x96-Strip-Wells

2340

10x96-Strip-Wells

4370