ELISA/assay

Rat Soluble Glycoprotein VI, SGPVI ELISA Kit

MBS703420

96 Strip Wells

850 USD

ELISA Kit

Rat Soluble Glycoprotein VI, SGPVI ELISA Kit

Soluble Glycoprotein VI (SGPVI)

Rat Soluble Glycoprotein VI (SGPVI) ELISA KIT; Soluble Glycoprotein VI (SGPVI)

sGPVI

Rat

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Detection Range: 1.56pg/ml-100 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 pg/ml

Sample Volume: 50-100ul

Introduction: GlycoproteinVI also known as GPVI is a glycoprotein receptor for collagen which is expressed in platelets. Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets.Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sGPVI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sGPVI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sGPVI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sGPVI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD