ELISA/assay

Pig Leptin, LEP ELISA Kit

MBS703419

48-Strip-Wells

335 USD

ELISA Kit

Pig Leptin, LEP ELISA Kit

leptin

Pig Leptin; LEP ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin

leptin; Leptin; leptin; obese; obesity factor; Obesity factor

LEP

LEP; LEP; OB; OBS; OB; OBS

LEP_PIG

Pig

167

This assay has high sensitivity and excellent specificity for detection of pig LEP. No significant cross-reactivity or interference between pig LEP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.25 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of pig LEP is typically less than 0.125 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest pig LEP concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for LEP and Horseradish Peroxidase (HRP) conjugated LEP. The competitive inhibition reaction is launched between with HRP labeled LEP and unlabeled LEP with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.

55741433

NP_999005.1

NM_213840.1

Q29406

18,661 Da

AMPK Signaling Pathway (989938); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (84504); Adipocytokine Signaling Pathway (505); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1014043); Incretin Synthesis, Secretion, And Inactivation Pathway (1014416); Jak-STAT Signaling Pathway (84489); Jak-STAT Signaling Pathway (488)

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

48-Strip-Wells

335 USD

96-Strip-Wells

460

5x96-Strip-Wells

1725

10x96-Strip-Wells

3200