catalog number :
MBS703413
products type :
ELISA Kit
products full name :
mouse acetyl-CoA ELISA Kit
products short name :
acetyl-CoA
products name syn :
mouse acetyl-CoA ELISA Kit; acetyl-CoA
other names :
acetyl coA, partial; Acetyl coA; Acetyl coA
uniprot entry name :
Q9UAR5_CRYPV
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse Acetyl-CoA. No significant cross-reactivity or interference between mouse Acetyl-CoA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml-50 ng/ml. Sensitivity: The minimum detectable dose of mouse Acetyl-CoA is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Acetyl-CoA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Acetyl-CoA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Acetyl-CoA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Acetyl-CoA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAD32537.1
ncbi mol weight :
12,156 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells