catalog number :
MBS703403
products type :
ELISA Kit
products full name :
Human beta defensins 2 ELISA Kit
products short name :
beta defensins 2
products name syn :
Human b defensins 2 ELISA Kit
other names :
beta-defensin 4B; Beta-defensin 4A; beta-defensin 4B; defensin, beta 4, pseudogene; defensin, beta 4B; Beta-defensin 2; BD-2; hBD-2; Defensin, beta 2; Skin-antimicrobial peptide 1; SAP1
products gene name :
DEFB4
other gene names :
DEFB4B; DEFB4A; DEFB4P; DEFB102; DEFB2; DEFB4 DEFB4B ; BD-2; hBD-2; SAP1
uniprot entry name :
DFB4A_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human beta-defensins 2. No significant cross-reactivity or interference between human beta-defensins 2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, urine, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001192195.1
ncbi gb acc num :
NM_001205266.1
ncbi mol weight :
7,038 Da
ncbi summary :
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Oct 2014]
uniprot summary :
Function: Has antibacterial activity Reviewed prediction.
size4 :
10x96-Strip-Wells